Effect of the Viscosity and Application Time of Different Phosphoric Acids on the Bond Strength and Nanoleakage of Fiber Posts to Root Dentin
To evaluate the effect of application time and viscosity of meta-phosphoric acid (MPA) and ortho-phosphoric acid (OPA) on the push-out resin-dentin bond strength (PBS) and nanoleakage (NL) at the adhesive-root dentin interface. Ninety-six roots of premolars were endodontically prepared and randomly assigned into groups according to the (i) acid (OPA and MPA), (ii) viscosity (gel and liquid), and (iii) application time (7 and 15 seconds). Fiber posts were cemented to the roots, which were then transversally sectioned into serial slices. The slices (cervical, medium, and apical) were subjected to PBS or NL. Analysis of the dentin etching pattern was performed using scanning electron microscopy (SEM). The PBS (MPa) and NL (%) data were subjected to three-way repeated-measures analysis of variance (ANOVA) and Tukey’s test (α=0.05). The cross-product interaction was significant for both PBS and NL (p<0.001). No significant difference in the PBS was observed with the use of OPA compared to MPA (p>0.05). Higher PBS values were observed in the groups etched for 15 seconds compared to 7 seconds, regardless of the acid, viscosity, or root third (p<0.05). Lower NL was observed for MPA etching when compared to OPA etching (p<0.05), and these values were not affected by increasing the application time or acid viscosity (p>0.05). A more pronounced etching pattern was observed with OPA than MPA regardless of the acid viscosity. The use of 40% meta-phosphoric acid promoted adequate bond strength without increasing dentin demineralization or void spaces in the hybrid layer.SUMMARY
Purpose
Methods and Materials
Results
Conclusion

Representative SEM micrographs for each experimental group in the cervical and apical area. Independent of the viscosity, ortho-phosphoric acid (OPA; capital letters) produced major silver deposits compared to meta-phosphoric acid (MPA; lower letters). Increased silver nitrate deposition was observed at the bottom of the hybrid layer (white hands) when OPA was applied. However, a small amount of discontinuous silver deposit can be seen in the MPA groups (red hands).

Representative SEM micrographs of dentin surfaces after conditioning with ortho-phosphoric acid (OPA; capital letters) and meta-phosphoric acid (MPA; lower letters) in the cervical and apical area. Acid etching with OPA totally removed the smear layer on the surface of the dentin tubules, and the tubular diameter was significantly enlarged (white hands) when compared to MPA, independent of the acid viscosity. Etching with MPA preserved the tubular diameter and the presence of the smear layer in the lumen of the tubule (red hands). When OPA was applied for different durations, some regions of the intertubular area with signs of erosion were observed with 15 seconds’ application (white asterisk) compared with 7 seconds’ application. In comparison, no significant difference was observed for MPA, despite the very superficial intertubular dentin etching pattern observed with 7 seconds’ application (red asterisks) compared to 15 seconds’ application. SEM images of the middle third are not presented in this figure because no significant difference in the conditioning pattern was observed when the cervical and medium thirds of the root canal were etched with OPA and MPA.
Contributor Notes
Clinical Relevance
Meta-phosphoric acids could be an alternative to ortho-phosphoric acids as a means to achieve a more stable hybrid layer when bonding fiber posts.